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The monolayer cells were subsequently lysed with 200 L 0.1% Triton X-100 for 20 min at room temperature, and were then diluted with PBS and plated on TSB-agar plates to determine the number of intracellular bacteria (Colony-Forming Units, CFU)

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Each device delivers two heating modes, standard and boost, with ramp-up times of 20 seconds and 10 seconds, respectively, and up to 20 sessions per charge