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[67] The campaign introduced 6 media campaigns (entitled 'Artery', 'Lung', 'Tumour', 'Brain', 'Eye' and 'Tar') between the years of 1997 and 2000, intending to appeal to people of lower socioeconomic status by illustrating that individuals in this cross section of society had the highest smoking rates and suffered the highest levels of smoking related disease

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Artist's Description Close up photograph of a wet Havana Corojo tobacco leaf

On the next day, the media was changed, and the adherent cells were treated with serial concentrations of two different smoke extracts, including 100, 1.0, 0.1, 0.01, and 0.001 M of each extract, named tobacco extract powder (TE-p) and e-cigarette extract liquid vape (e-Cig-LV)