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Subsequently, the ATP6AP1L coding sequence was amplified from the cDNA of T47D cells and inserted into the FastDigest KpnI/FastDigest XbaI (Thermo Fisher) linearized pcDNA3.1-GST DAF and pcDNA3.1-GST CEAM7 vectors using T4 DNA ligase (EL0011, Thermo Fisher)

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64 : 259–264 Akhand AA, Du J, Liu W, Hossain K, Miyata T, Nagase F, Kato M, Suzuki H and Nakashima I (2002) Redox-linked cell surface-oriented signaling for T-cell death