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Microscopically, controls presented a diffuse edema of interlobar septe, interlobular septe, interacinal spaces, focal expansion of intercellular spaces, a diffuse occurrence of 5–10 necrotic acinar cells and a focal occurrence of 11–16 necrotic cells/HPF, up to four foci of hemorrhage, up to six foci of fat necrosis, and perivascular infiltration of 6–10 leukocytes/HPF in the 4.30 h–5 h assessment period (Figure 6)

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As such, we generated a mutant D23A at the predicted allosteric site