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Pharmacodynamic study in DIO mice Female DIO mice with a body weight approximately 22 g received tail vein injections of either saline (Saline), scExe4 donor plasmid loaded LNP only (Donor), or scExe4 donor plasmid loaded LNP plus Cas9 plasmid loaded LNP (Donor+Cas9)

Insert the needle at a 90-degree angle to the skin, but avoid going too deep Administer the medication slowly and steadily to minimize tissue trauma Rotate the injection site

This helps preserve vital skeletal muscle and maintains a healthy metabolic rate

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Dispose of the used syringe immediately in a sharps container

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