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Once the optical density (OD 600 ) reached approximately 0.6, the culture was induced with 1 mM IPTG (Isopropyl -D-1-thiogalactopyranoside) and incubated for an additional 23 h at 37 C in a shaker

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Identification and biological evaluation of 4-(3-trifluoromethylpyridin-2-yl)piperazine-1-carboxylic acid (5-trifluoromethylpyridin-2-yl)amide, a high affinity TRPV1 (VR1) vanilloid receptor antagonist

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