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doi: 10.1038/ijo.2013.83

The antidiabetic drug metformin activates the AMP-activated protein kinase cascade via an adenine nucleotide-independent mechanism

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RAW264.7 cells were seeded into six-well plates and incubated at 37C with 5% CO 2 , after 24 h of adhesion, were treated with GLP-1 (10, 20, and 50 mol/L) for 4 h and then co-exposed to LPS (1 g/mL) for 24 h