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Materials and methods: We performed high-throughput screening of a compound library in MIN6 cells expressing pHluorin-LC3-mCherry reporter, with which pHluorin/mCherry fluorescence ratio decreases in response to an increase in autophagic flux, to determine agents upregulating the autophagic flux

The addition of the C20 fatty diacid conjugation plays a key role in studies exploring the effects of peptide modifications on performance and stability within biological matrices and model systems