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Notably, the C-terminal residue of GIP(1-42) is Q 64 , so PC1/3 cleavage after R 65 suggests that there is another proteolytic event which removes R 65 to generate the 42 amino acid polypeptide, likely to be carboxypeptidase E mediated in analogy to general prohormone processing [223]

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d) sequential coupling of Fmoc-Arg(Pbf)-OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc- Trp(Boc)-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Lys(C18diacidmono-t-butyl-Y-Glu(AEEA-AEEA)-OtBu)-OH, Fmoc-Ala-OH, Fmoc-Ala-OH, Fmoc-Gln(Trt)-OH to the obtained resin in step-c) in presence of a coupling agent