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Build practical routines: three to five sessions per week, mixing aerobic and resistance work at moderate intensity, and include daily short walks to limit prolonged sitting

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After washing three times with 1x PTW/0.5% BSA solution for at least 30 min (10-min interval), the dissected gonads were stained with DAPI solution (100 ng/mL) for 10 min at room temperature and were washed with 1x PTW/0.5% BSA solution three times