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Although HFpEF shares symptomatic similarities with heart failure with reduced ejection fraction (HFrEF), it is characterized by distinct pathophysiological mechanisms, and transition from HFpEF to HFrEF is exceedingly rare [3]
Equal amounts of protein (50-60 g per lane) were separated by 10% SDS-PAGE and then transferred to 0.22-mm PVDF membranes (MilliporeSigma)
