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The crude viral lysate was initially concentrated by a brief two-tier CsCl gradient centrifugation for 3 h and then the vector fraction was dialyzed in MHA buffer (3.3 mM morpholinoethanesulfonic acid [MES], 3.3 mM HEPES [pH 7.5], 3.3 mM sodium acetate)
The shorter half-life necessitates more frequent administration but allows faster dose adjustments

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Direct head-to-head comparisons are not possible because the trials differed in duration, population and study design, but reporting outcomes side-by-side provides context

This step reflects DRAPs ongoing efforts to align local product standards with internationally recognized pharmacopoeial references, ensuring consistency, quality, and safety of drug products available in Pakistan