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This has been achieved through techniques such as peptide sequence modification, peptide lipidation, albumin fusion, and Fc fusion

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Enzymatic Digestion Although trypsin is the standard protease, Semaglutides structure required alternative enzymes due to limited cleavage sites: Chymotrypsin was chosen to cleave at aromatic residues (e.g., Phe, Tyr, Trp)

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Higher preoperative BMI, radical tumor resection, larger preoperative tumor size, hypothalamic tumor invasion, adamantinomatous subtype, and familial predisposition to obesity are cited as factors that increase the risk of HO [49, 52, 53]

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