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The antibodies used in WB were: INSULIN (1:1000, ab181547, Abcam), PPAR (1:1000, 74076 S, CST), UCP2 (1:1000, 89326 S, CST), PDX1 (1:1000, ab219207, Abcam), MAFA (1:1000, 7937 S, CST), IRS1 (1:1000, 2390 S, CST), GCK (1:1000, 19666-1-AP, Proteintech), -ACTIN (1:1000, PM053, MBL) and -TUBULIN (1:1000, 2128 S, CST)

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This dual action provides approximately 3x greater fat metabolism through the GIP pathway

Gastrointestinal side effects, including nausea, occur in approximately one-quarter of patients starting semaglutide therapy

Biomarker shifts (e.g., modest reductions in inflammatory and neurodegeneration markers) were observed but did not translate into clinical benefit