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Metabolite pathway sets were downloaded from the Small Molecule Pathway Database (SMPDB) (71), and metabolite set enrichment was tested using the limma ROAST method (72)

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Water retention patterns, appetite changes, and metabolic rate fluctuations tied to the menstrual cycle can create a less smooth weight loss curve

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When the medication is removed, appetite regulation returns to its previous baseline

As hepatocytes do not express the GLP-1R, the benefits of semaglutide may be secondary to weight loss or indirect reflecting local or systemic actions of semaglutide to improve hepatic metabolism and inflammation (Figure 1B)