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Description

a) reacting Boc-His(PG)-Aib-OH with H-Glu(OtBu)-Gly-Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)- Asp(OtBu)-Val-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-Gln(Trt)-Ala-Ala- Lys(Boc)-Glu(OtBu)-Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Resin to provide Boc-His(PG)-Aib-Glu(OtBu)-Gly-Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)- Asp(OtBu)-Val-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-Gln(Trt)-Ala-Ala- Lys(Boc)-Glu(OtBu)-Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(Pbf)-Gly-Arg(Pbf)-Gly-Resin (Resin bound Semaglutide backbone), b) converting the compound obtained in step a) to Semaglutide

Some evidence suggests nasal delivery may be more effective than sublingual for certain peptides due to the different characteristics of nasal epithelial tissue

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Chromatography was achieved using a 50 mm C18 column with a mobile phase consisting of formic acid and an organic solvent, with a total run time of 13.0 minutes