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They found no evidence of increased psychiatric symptoms or suicidality
doi: 10.1038/s41574-019-0168-8 169 MartinCPassilly-DegracePChevrotMAncelDSparksSMDruckerDJet al
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Zinc competes with copper for absorption, so prolonged zinc supplementation should include a small amount of copper (1 to 2 mg) to prevent secondary copper deficiency

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Western blot Equal amounts (50 g) of RINm5F, INS-1, and MIN6 cell lysate were analyzed by Western blotting with rabbit anti-AMPK monoclonal antibody#ab32047 (Epitomics Technology, Berlingame, CA), rabbit anti-phospho-AMPK(Thr172) monoclonal antibody #2531 (Cell Signaling Technology, Beverly, MA), rabbit anti-PARP polyclonal antibody#9542S, anti-cleaved caspase-3, phospho-CREB/CREB, PDX1, phospho Akt/Akt and GAPDH (Cell Signaling Technology, Beverly, MA), respectively
