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For DPP-4 activity assays, blood was collected before and 30 minutes or 1 hour after olive oil gavage, and activity was assessed using a fluorometric assay (substrate: 10 mM H-Gly-Pro-AMC HBr [Bachem, I-1225], standard: AMC [Bachem, Q-1025])

The study found increased risk in both patients with type 2 diabetes and patients using semaglutide for overweight or obesity, but the authors noted that the study was observational and could not prove that semaglutide directly caused NAION