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There are a multitude of successful differentiation protocols, mostly based on a combination of cell culture medium with different growth supplements, nicotinamide and beta-mercaptoethanol with an average protocol duration of 17 days (Table 3)

nIs590 [fat-7p::fat-7::GFP + lin15(+)] V ] and IU830 [IU653 [ mog-3(q74)/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III)]

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