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Leptin regulates gastrointestinal satiation signals, such as gastric distension, CCK or glucagon-like-peptide-1 (GLP-1) by acting on LepR in mNTS/AP neurons [56], [57], [58], [59]

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For example, at the cellular level, primary neuronal cells from GLP-1R KO and WT mice could be cultured, and the levels of cAMP and changes in downstream phosphorylation of PKA and CREB signaling pathway after GLP-1R agonist treatment could be tested

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