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Here is what I would actually do if I were in this position

Plaintiffs, however, argued that optic neuropathy involves different medical questions, scientific evidence, and expert testimony

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Following incubation, cells were washed three times with ice-cold PBS and lysed by addition of 50 L lysis buffer (PBS supplemented with 20 mM Tris-HCl, 1% Triton X-100, pH 7.4)
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