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Description
This fat accumulation can cause increased inflammation
Time-resolved fluorescence was quantitated with a Pherastar FSX multilabel reader (BMG Labtech)
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Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
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After 24 h incubation, fibroblasts were fixed with 4% (w/v) paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with QuickBlock Blocking Buffer (Beyotime, Shanghai, China), and incubated with ASC antibody (1:400, Cell Signaling Technology, Danvers, MA, USA, 67824s) at 4 overnight
