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Calmodulin-dependent protein kinase kinase-beta is an alternative upstream kinase for AMP-activated protein kinase

Heart nuclei were isolated using a lysis buffer consisting of 0.25M sucrose, 10mM Tris-Hcl pH 7.5, 25mM KCl, 5mM MgCl2, 45uM Actinomycin D, supplemented with 1X protease inhibitor (G6521, Promega), 0.4U/uL RNasin Ribonuclease Inhibitor (N2515, Promega), 0.2U/ul SuperaseIn (AM2694, ThermoFisher)

The foods to avoid are everything, at least for the 30-minute window before dosing

Sequence: Y-Aib-E-G-T-F-T-S-D-Y-S-I-L-L-D-K-Aib-A-Q-Aib-A-F-I-E-Y-L-L-E-G-G-P-S-S-G-A-P-P-P-S-NH2

Fardet A, Boirie Y

Pair these foods with vitamin C-rich items like citrus fruits and bell peppers to enhance absorption