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Bacterial culture was pelleted at 2,500 rpm for 30 minutes and resuspended in lysis buffer (0.1 M NaCl, 20 mM Tris-HCl, pH 8.0, 1 mM DTT, 1X protease inhibitor cocktail) and 0.35 mg/mL lysozyme added for 30 minutes on ice

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Significant mood changes Mood changes and worsening depression, or any thoughts of self-harm that develop or worsen after starting a GLP-1 medication should be discussed with a doctor promptly

Rapid caloric restriction can affect serotonin production, since the amino acid tryptophan (a serotonin precursor) comes from dietary protein

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