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Then, the 5-HT is successively converted into N-acetyl-5-hydroxytryptamine (NAS) and melatonin by arylalkylamine N-acetyltransferase (AANAT, EC: 2.3.1.87) and acetylserotonin O-methyltransferase (ASMT, EC: 2.1.1.4) (Figure 1)

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Individual experiences vary widely, and no specific timeline is guaranteed: Early weeks: Appetite suppression is often among the first effects people notice