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After DNA extraction and amplification, the PCR product was loaded on 1% agarose gels (UltraPure Agarose, Invitrogen, Thermo Fisher Scientific), then separated for 30 minutes at 150 V (Owl Easycast B2 Separating System), and the bands were visualized under a blue light transilluminator (UVP GelDoc-It2 Imager)

If something isnt working, say, constipation is unmanageable or nausea is too severe, your doctor might adjust the dose escalation, prescribe a concurrent medication, or in some cases consider an alternative weight management approach

The full publication is available here References: Baggio LL, Drucker DJ (2007) Biology of incretins: GLP-1 and GIP

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