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However, GLP-1 does not just act on the gut

Picked particles were extracted and 2D classified using RELION (version 3.0.7)

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The effect of substitution on binding affinity (GLP-1 radioligand competition IC 50 ) and potency (cAMP activity EC 50 ) was, however, much larger for Phe 28 (1300/1000-fold decrease in affinity/potency) and Ile 29 (93/27-fold reduction) compared with Leu 32 (17/twofold decrease), indicating that interactions of Phe 28 and Ile 29 with the conserved hydrophobic core of the ECD of GLP-1R are particularly important (Adelhorst et al., 1994)