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Acid reflux affects approximately 2030% of patients starting GLP-1 therapy, making it one of the most commonly reported gastrointestinal side effects alongside nausea

Blood samples were centrifuged at 3000 g for 15 min at ambient temperature to collect supernatant plasma

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After 24 h incubation, fibroblasts were fixed with 4% (w/v) paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with QuickBlock Blocking Buffer (Beyotime, Shanghai, China), and incubated with ASC antibody (1:400, Cell Signaling Technology, Danvers, MA, USA, 67824s) at 4 overnight
