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Many studies have also indicated that alcohol use increases the risk of common diseases, such as cardiovascular disease, liver disease, and cancers, which significantly contribute to mortality and morbidity rates

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Step b): Preparation of seed medium in seed fermenter: 500ml of seed medium was prepared in 2L Erlenmeyer flasks by using raw materials as mentioned in Table 2

It must motivate patients to share their experiences, symptoms, concerns, and treatment preferences openly and honestly

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