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In a rat model of subtotal nephrectomy, which mimics CKD, 7 weeks of twice-daily liraglutide treatment attenuates Ager expression in the kidney
All CRISPR alleles were verified by sequencing and outcrossed 24 times with wild type prior to analysis
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In different cell backgrounds, two distinct mechanisms of internalization have been observed, with both mechanisms dependent on dynamin (Widmann et al., 1995
For the extraction of total RNA, tissue samples were pulverized using GenoGrinder (ColeParmer), then homogenized in Tri Reagent (Molecular Research Center or Ambion by Life Technologies) using a MagNa Lyser or TissueLyser II system (Qiagen)
