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Philippe Halban, University of Geneva, Switzerland) were maintained in DMEM at 25 mM d-glucose supplemented with 15% FBS, 50 M -mercaptoethanol, and 1% penicillin/streptomycin 22
After stopping treatment, patients still need careful and continued treatment and monitoring to ensure that they do not rapidly regain the lost weight [13]

Loss of interest in previously enjoyable activities

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