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Semaglutide, tirzepatide, and retatrutide (Biosynth, Compton, UK, lot #s 0000042482, 0000054911, and 0000205204 respectively) were dissolved in 0.3% phosphate-buffered saline (PBS), which was adjusted to a pH of 8.0 using NaOH and injected subcutaneously (s.c.) at 1 ml/kg

Roczniki Panstwowego Zakladu Higieny, 72(1), 2128

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[13] For the liver side specifically, researchers use hepatocyte lipid-loading models (exposing primary human hepatocytes or HepG2 cells to fatty acids such as oleate and palmitate to induce steatosis in a dish), hepatic stellate-cell lines (for example LX-2) to study fibrogenic activation, and increasingly three-dimensional systems liver organoids, spheroids, and liver-on-a-chip devices that better reproduce cellcell interactions