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After growth and expansion of clones, genomic DNA was extracted from cells using standard protocols and fragments encompassing the CRISPR target sequences were amplified by PCR

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5 Ways We Built GLP-1 Support Differently 5 Ways We Built GLP-1 Support Differently 1

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We explored this further by depleting cells of both -arrestin isoforms to better represent the loss of recruitment of both -arrestins seen with exendin-phe1

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