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Perturbations of NAD(+) salvage systems impact mitochondrial function and energy homeostasis in mouse myoblasts and intact skeletal muscle

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In brief, cells were plated on glass coverslips and fixed with 4% formaldehyde and permeabilized with 0.1% Triton X100

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While holding the syringe with the newly attached needle pointing up, push the plunger up until a tiny drop of medicine is seen on the needle tip