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Protocols for fluorescence exposure of slices were controlled by software (Live Acquisition, TillVision), and analyses were conducted using Offline Analysis (TillVision)

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Their study demonstrated that enzymatic ligation proceeds efficiently under physiological pH and in the presence of 10% acetonitrile as a cosolvent, working effectively with both crude and purified fragments

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This is precisely why the titration protocol calls for a dose increase at week five