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On the day of the experiment, the culture medium was replaced with DMEM containing 5.6 mM glucose and 0.1% bovine serum albumin (BSA) and the cells were then serum starved for 3 hours while equilibrated in a tissue culture incubator
A measurable increase in GLP-1 levels doesnt automatically mean weight loss
Stratification was based on incidence of the race/ethnicity groups within each frame
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