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Rapid or drastic weight loss can lead to something called hypothalamic amenorrhea 2

Oxidation-sensitive residues: peptides containing Cysteine (C), Methionine (M), or Tryptophan (W) may be more prone to oxidation and should be handled with additional care

Given the emerging evidence, it is likely that IIH is triggered by systemic metabolic derangements that ultimately lead to disturbances in CSF dynamics

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After treating the sections at 37 C with binding buffer (Composition in mmol L 1 : HEPES 20, NaCl 150, MgCl 2 5, ethylene glycol-bis (-aminoethyl ether)- N , N , N ', N '-tetraacetic acid 1, and 0.1% BSA) for 60 min, we incubated the vagal branch with 15 nM 125 I-ghrelin or 15 nM 125 I-GLP-1 for 30 min

This connects to everything we cover in hormone balance