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Combining Fasting with GLP-1 Therapy for Optimal Results Some people combine intermittent fasting with prescription GLP-1 medication, though medical supervision is essential
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The supernatant was discarded, and the pellet resuspended in cold PBS + 1% Triton X-100 plus protease and phosphatase inhibitor cocktails, transferred to an Eppendorf tube and allowed to rotate for 30 minutes at 4C
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