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doi: 10.1038/379069a0 54 BarreraJGJonesKRHermanJPDAlessioDAWoodsSCSeeleyRJ

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Real-time qPCR was performed using the Light Cycler 480 system (Roche Diagnostics, Indianapolis, IN, USA) according to the manufacturers instructions under the following conditions: 15 min denaturation at 95 C followed by 40 cycles of 10 s at 95 C, 45 s at 60 C, and 10 s at 72 C