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Freezing and thawing causes protein denaturation that destroys the peptide structure

D.BinkleyN.CzerwinskiE.FerrariS.et al (2016)
Bianchi R, Cervellini I, Porretta-Serapiglia C, et al
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AMPK Inhibits PARP1 Activity to Attenuate Cholesterol-Induced Toxicity The potential attenuation of cholesterol-induced toxicity through PARP-1 activity by AMPK activation was further investigated

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