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doi: 10.1080/21623945.2020.1870060

Understanding these mechanisms helps explain why some patients experience oral dryness when taking semaglutide

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Agonist dissociation was monitored immediately after washing in 10 M exendin(9-39) by TR-FRET at 37 C, with 50 s delay and 300 s integration time, and binding quantified as 520 nm over 620 nm signal after nonspecific binding subtraction with excess (10 M) unlabeled agonist
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