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Cryo-EM data acquisition The purified sample (3 L) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific) set at 100% humidity and 4C
Abdel-Moneim A, Bakery HH, Allam G

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