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Cells were imaged 24 hours after seeding in RPMI-1640 buffer without phenol red 100 nM exendin-4 by time-lapse microscopy every 12 seconds for 11 minutes in a 37 C heated chamber using a Leica Stellaris 8 inverted confocal microscope with a 40/1.2 NA oil immersion objective with excitation wavelengths of 440 and 580 nm and emission wavelength of 620 nm

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Circulating GLP-1 levels also correlate with severity of critical illnesses (5, 10), sepsis (11), end-stage kidney disease (11), respiratory failure (12), and intestinal ischemia-reperfusion injury (6)

GLP-1 injections were also shown to improve myocardial tolerance to stress induced ischemia at peak activity and 30 min post-activity [73]

GADD34, growth arrest and DNA damage inducible gene 34

Annex 1 compliance remains a key priority for companies working in sterile drug product manufacturing