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Importantly, the effect is most pronounced in proximal colon cancers, a region historically less protected by screening alone

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Cell culture INS-1 832/3 cells, a rat insulinoma subline with preserved incretin responses (64), and derivative INS-1 832/3 cells with endogenous rat GLP-1R deleted by CRISPR/Cas9 [INS-1 832/3 GLP-1R KO (27)], were grown in RPMI-1640 medium with 2 mM L-glutamine and 11 mM D-glucose, supplemented with 10% FBS, 1% p/s, 10 mM HEPES solution (H3537, Sigma-Aldrich), 1 mM sodium pyruvate (11360070, Thermo Fisher Scientific) and 0.05 mM -mercaptoethanol (M3148, Sigma Aldrich) in a humidified incubator at 37C and 5% CO 2

Tirzepatide, the active ingredient behind the popular weight loss drug Zepbound and the diabetes drug Mounjaro, has more tricks up its sleeve

Diabetes Care 39:e199e200 Kelly RP, Garhyan P, Raddad E et al (2015) Short-term administration of the glucagon receptor antagonist LY2409021 lowers blood glucose in healthy people and in those with type 2 diabetes