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Clin J Am Soc Nephrol 2020;15:46573

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For example, in the lateral hypothalamus exendin-4 is less potent at inducing anorexic downstream mediators like neurotensin or IL-6 and at suppressing downstream orexigenic mediators (eg, orexin) when tested in estrous cycle phases in which estrogen levels are low (33, 47)

doi: 10.1111/j.1463-1326.2012.01618.x

After 1.5 h incubation at RT, the membrane was solubilized in the buffer above supplemented with 0.5% (w/v) LMNG and 0.1% (w/v) CHS for 2 h at 4 C