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Description
Eur J Intern Med

RNA isolation and qRTPCR analysis Total RNA was extracted using TRIzol (Takara Bio, Tokyo, Japan) and quantitative reverse transcription polymerase chain reaction (qRTPCR) was performed using Hieff qPCR SYBR Green Master Mix (Vazyme, Nanjing, China) according to the manufacturers instructions

Drinking large amounts with food dilutes digestive enzymes and adds volume to an already slow-emptying stomach, increasing nausea and bloating

Qi L, Ke L, Liu X, et al

A case report
After 24 h at 37 C, the migrating cells were fixed, stained, and quantified under an optical microscope
