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Rectal body temperature Conscious mice were restrained and a high-precision thermometer (BIO-TK8851, Bioseblab) was carefully inserted half-way into the rectum

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The choice for K = 10 was based on a combination of minimizing cross-validation errors for admixture analysis, as well as minimizing the Frobenius distance of each ADMIXTURE matrix K with the matrix outputted by Genetic Relationship and Fingerprinting

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and North Carolina briefly removed then reinstated coverage