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One potential strategy involves engineering brain-targeted NPs designed to release exenatide within the initial 72 h post-stroke, with the aim of achieving optimal neuroprotection [304306]

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When L6 cells reached 70%80% confluence, DMEM containing 2% FBS was used to induce L6 cells to differentiate into myotubes for 56 days

After full recovery, correct cannulation was tested by observing water drinking responsiveness after infusion of 1 l human angiotensin II (Sigma-Aldrich, A9525) at a concentration of 24 M in artificial cerebrospinal fluid (distilled water with 125 mM NaCl, 2.5 mM KCl, 2.6 mM NaHCO 3 , 1.25 mM NaH 2 PO 4 2H 2 O, 25 mM d-glucose monohydrate, 1 mM MgCl 2 , 2 mM CaCl 2 )
