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Lejeune S, et al
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The optical density of each well was determined with the GloMax-Multi Detection System (Promega Corp., Madison, WI Part #TM297) and a regression model was applied to calculate total (i.e., acyl + des-acyl) ghrelin concentrations
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Do your prescribed number of sprays (1, 2, or 3 per day depending on your protocol)
Injection technique tips specific to the 7.5 mg volume The volume you inject affects the injection experience
