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Upon initiation of the perifusion experiment, KRPH with 2.7 mM glucose + 1% BSA was perifused at a rate of 200 L/min for 24 minutes, followed by KRPH with 2.7 mM glucose +0.1% BSA, to provide an equilibration period for the islets

[DOI] [PubMed] [Google Scholar] 149.Jastreboff AM, Kaplan LM, Fras JP, Wu Q, Du Y, et al

Details on the isolation of mouse caSMCs and neonatal ventricular CMs, as well as cell culture methods for human caSMCs, caECs, hESC-CMs, and the HL-1 cell line, including drug treatment regimens, are provided in the Supplemental Methods

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Choosing the best day for your tirzepatide injection often means selecting a day where you can rest if side effects occur